A kit for performing twenty cDNA syntheses from nanogram amounts of total or poly A+ RNA. The kit utilizes our SMART (Switching Mechanism At 5' end of RNA Template) technology coupled with PCR amplification to generate high yields of full-length double-stranded cDNA suitable for various applications, including Clontech PCR-Select cDNA subtraction, nondirectional cloning, and preparation of complex cDNA probes. The SMARTer PCR cDNA Synthesis Kit includes the same components as the original SMART PCR cDNA Synthesis Kit plus an improved SMARTer II A oligo and SMARTScribe Reverse Transcriptase, which provide higher specificity and increased yield.

Overview

• Start with as little as 1–2 ng of total RNA
• Specific enrichment for full-length cDNA
• Optimized protocol for retaining true gene representation
• Simplified protocol without DNase treatment or a separate adapter-ligation step

Applications

• Cloning and library construction
• Probe generation
• Subtractive hybridization
• Next-generation sequencing
• Virtual northerns
• Template generation for PCR/qPCR