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639503-Titanium One-Step RT-PCR Kit
Brand: Clontech
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Titanium One-Step RT-PCR Kit SKU:639503 30 Rxns
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The Titanium One-Step RT-PCR Kit is designed for the reverse transcription of total or poly A+ RNA and the subsequent PCR amplification of a target cDNA in a single reaction tube. The included enzyme mix contains a highly purified Moloney murine leukemia virus (MMLV) reverse transcriptase for first-strand cDNA synthesis, and Titanium Taq DNA Polymerase for PCR amplification. Titanium Taq DNA Polymerase is a blend of a 5'-exonuclease deficient, thermostable DNA polymerase, and TaqStart Antibody for automatic hot start PCR. Reagents for purifying RNA are not supplied.

 

Overview

  • Allows qualitative analysis of gene expression from multiple samples

  • Produces results from as little as 10 pg of total RNA

  • Provided in a convenient single-tube format
  • Easy-to-use one-step format minimizes the risk of cross contamination

 

 

Applications

  • One-step RT-PCR
  • Endpoint RT-PCR
  • Gene expression analysis
  • First-strand cDNA synthesis
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