PrimeScript RT Reagent Kit (Perfect Real Time): generate cDNA for real-time PCR

PrimeScript RT Reagent Kit is designed to perform reverse transcription optimized for real-time RT-PCR. It uses PrimeScript Reverse Transcriptase, which features excellent extension. The kit makes fast, efficient cDNA template synthesis for real-time PCR possible. This kit's protocol is simple and suitable for high throughput analysis. It can be used in combination with real-time PCR reagents such as TB Green Premix Ex Taq II (Tli RNaseH Plus) (Cat. #RR820A/B), TB Green Fast qPCR Mix (Cat. #RR430A/B), TB Green Premix Ex Taq (Tli RNaseH Pluse) (Cat. #RR420A/B), or Probe qPCR Mix (Cat. #RR391A/B) for two step real-time RT-PCR. The optimized protocol for assay can be selected in each assay condition using either TB Green or a probe.

Overview

• Allows fast, efficient synthesis of cDNA for real-time PCR. This kit is designed for two-step real-time RT-PCR.
• Includes random 6-mers and oligo dT primer for use as reverse transcription primers. The reaction can be performed using one or a mixture of these two primer types; alternately, gene-specific primers may be used.
• Includes EASY Dilution (for Real Time PCR) when generating reference standards for a qPCR standard curve. This reagent facilitates higher accuracy at lower standard concentrations.

Applications

• cDNA synthesis for real-time PCR