Cell Analysis Overview

RWD C100-Pro Automatic Cell Counter Realizes Analysis With Multiple Application Scenarios

Cell Count Analysis: Suitable for counting cells or particles with a diameter of 4-60μm, such as most cell lines, stem cells, primary cells, etc.

Cell Viability Analysis: When testing cell viability in trypan blue mode, after staining, live cells will be circled in green, and dead cells will be circled in red.

Cell Size Analysis: In cell research, cell size is a key feature, which is often measured in cell transfection, drug testing and cell viability.

After counting, it will count the average diameter of the cells in the sample and automatically generate a histogram.

Cell Fluorescence Analysis:

Detect Cell Transfection Efficiency (GFP, RFP):
When transfecting cells with the target gene, the transfection efficiency needs to be evaluated. In general, the transfected plasmid has the sequence encoding the fluorescent protein as the reporter gene, so the successfully transfected cell should be positive for the fluorescent protein. In addition, using a fluorescence microscope or a flow cytometer is a commonly used method for transfection efficiency analysis in laboratories, but both have the disadvantages of complicated operation and high cost. Using the fluorescence analysis function of C100, equipped with the corresponding fluorescence module, can realize the analysis of the expression of GFP, RFP, and other fluorescent proteins, and automatically count them, so as to quickly, simply, and efficiently obtain the positiverate of target gene expression.

Cell Fluorescence Analysis:

Cell Viability Identification (AO/PI Dual Fluorescence Method).
In addition to trypan blue staining, AO/PI dual fluorescence staining has become popular as a more accurate live cell counting method and is widely used for primary cell counting.

Advantages

FDA 21 CFR Part11 compliance

Ensuring data security with account login and account permissions setting.

Ensuring data integrity with electronic signature and electronic recording.

Smart cell recognition

Clustered cells can be identified and counted separately. In trypan blue mode, the counting result will show the proportion of live cells and dead cells.

The results of HEK293 cell line and mouse primary macrophage count showed:

C100 counter has a smaller standard deviation of multiple counts.

The actual measured value and the theoretical value have a slight deviation in a great linear relationship.

The CV value of manual counting can reach more than 10%, while the CV value of the C100 counting result is much lower than 5%.

Fast cell counting in 9 seconds

Compared to the hemocytometer, it can save you more than 24hrs within a month when counting 10 samples per day

It takes about 5 minutes to use a hemocytometer for manual cell counting, while C100-SE/C100 only takes 9s. Counting ten samples per day can save more than 24 hours a month.

User-friendly operation

Touchscreen with built-in user guide interfaces ensures easy operation.

Compact design

Small size fits into different placements.

Removable modular FL cubes allow easy switches among various scenes.

Specification

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