Product name

HIF1a + GLUT1 Hypoxia Response Human Flow Cytometry Kit

Detection method

Fluorescent

Sample type

Adherent cells, Suspension cells

Assay type

Direct

Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Rat

Product overview

Hypoxia and the cellular response to hypoxic environment are central topics in studies of metabolism, cancer progression and development and stem cells. A key player is the transcription factor HIF1 alpha (hypoxia inducible factor 1 alpha) which is stabilized at the protein level in response to decreased oxygen tension. HIF1 alpha then promotes transcription of a number of factors that alters cellular physiology. This Hypoxic Response Human Flow Cytometry Kit provides duplexed measurements of the transcription factor HIF1 alpha and the HIF1 alpha responsive protein GLUT1.

Secondary antibodies are not included with this kit and must be purchased separately.

Positive control: DFO, ab120727 (to be bought separately).

Platform

Flow cytometer