• Product name

  Human CD40 knockout U-2 OS cell line

• Parental Cell Line

  U-2 OS

• Organism

  Human

• Mutation description

  Knockout achieved by CRISPR/Cas9; X = 1 bp insertion, 2 bp insertion; Frameshift: 99.09%

• Passage number

  <20

• Knockout validation

  Immunocytochemistry (ICC), Next Generation Sequencing (NGS), Western Blot (WB)

• Tested applications

  Suitable for: WB, ICC

• Biosafety level

  1

• General notes

  Recommended control: Human wild-type U-2 OS cell line (ab263976). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.

           Cryopreservation cell medium: Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

           Culture medium: McCoY5a + 10% FBS

           Initial handling guidelines: Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.