Product name

Human MAPK1 (ERK2) knockout HeLa cell line

Parental Cell Line

HeLa

Organism

Human

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2

Passage number

<20

Knockout validation

Immunocytochemistry (ICC), Sanger Sequencing, Western Blot (WB)

Tested applications

Suitable for: ICC/IF, WB

Biosafety level

2

General notes

Recommended control: Human wild-type HeLa cell line (ab255448). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.

Cryopreservation cell medium: Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Culture medium: DMEM (High Glucose) + 10% FBS

Initial handling guidelines: Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.