TaKaRa MiniBEST Plasmid Purification Kit Ver.4.0 is designed for rapid purification of plasmid DNA from a small amount of bacterial cultures. The kit is based on SDS-alkaline lysis of bacterial cells in combination with selective binding of plasmid DNA to the silica membrane of Spin Column. The protocol provides a simple method to achieve the rapid isolation of highly pure plasmid DNA and the whole procedure can be finished within 1 hour. 1 - 20 μg highly pure plasmid DNA (OD260/OD280 = 1.8 - 2.0) can be obtained from 1 - 4 ml cultured bacterial cells. The procedure is unnecessary for the phenol extraction and ethanol precipitation. The purified plasmid DNA can be dissolved in Tris buffer or water and used directly for many downstream applications, such as DNA sequencing, transformation, in vitro transcription and restriction digestion and other enzymatic reactions.